Since the publication of the first edition in 1983, several new and exciting developments have taken place in the field of plant tissue culture, which forms a major component of what is now called plant biotechnology. The revised edition presents updated information on theoretical, practical and applied aspects of plant tissue culture. Each chapter has been thoroughly revised and, as before, is written in lucid language, includes relevant media protocols, and is profusely illustrated with self-explanatory diagrams and original photographs. This book includes three new chapters: "Variant selection", "Genetic Engineering" and "Production of Industrial Compounds" and contains a complete bibliography and a glossary of terms commonly used in tissue culture literature.This updated version proves to be an excellent text for undergraduate, postgraduate students and teachers in various fields of plant sciences and a useful reference book for those interested in the application of any aspect of this aseptic technology.